FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11403052
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA294470.2582378822792354No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT154700.13566543413114313No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT130380.11433781061421101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG14450.026.9912095
GTATCGA19850.025.8034694
CCCGTAT23400.024.781421
CCCGTCG23600.023.37281
TATCGAA23150.022.3282035
ACCGCGT7900.020.2238526
GTCGAGG19700.020.036656
AGACCGC8700.018.904314
ACGACTT19950.018.4326861
CCGCGTT8700.018.3641077
GTTCGAC18400.018.2677928-29
TCGACCG18550.017.9954930-31
TTACGAC6550.017.9353895
GTCGGTA7600.017.369261
AGTTTAC206600.017.35662794
TCGAGGG23050.017.328397
CGGCATG43500.017.2840183
CGATCCG19450.016.79338582-83
ACGTACG11400.016.69557252-53
GTTACGA7050.016.6633764
Produced by FastQC (version 0.11.5)