FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11398154
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA297580.26107736393103653No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT156670.13745208215295215No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT135500.11887889916209239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA17950.027.2257864
CCCGTAT21100.025.9207121
CCCGTCG25200.024.3228361
TATCGAA20950.024.0000025
ACGACTT20450.022.825131
TGTCGAG15500.022.7374135
TTACGAC6850.021.2658445
TCGACCG21200.020.8393230-31
CGATCCG20800.020.78724982-83
CGCGTAT1605.2879528E-520.6276361
GTTCGAC22100.019.98960528-29
CGTTTAA14550.019.377595
GTCGAGG18200.019.3642836
AAGTTCG23550.018.45968426-27
GTTACGA7700.018.3080484
ACCGCGT7450.018.2916646
CTTCGCG20300.018.2870046
TTCGCGC20300.018.2869227
CGGCATG42650.017.6283843
GCGTTTA15350.017.4492994
Produced by FastQC (version 0.11.5)