FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11438591
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC226010.19758552430102624No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG162660.14220282900227835No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA145010.1267726068708987No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT130330.11393885837862373No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT126740.1108003599394366No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG125980.11013594244256132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC38750.043.4856451
CGACCGT14900.039.8032841
ACCGTTG17000.035.384213
ACGTGCG50900.032.95938
GACCGTT18300.032.665772
TAGCATA58100.030.9777729
CCAGTAG55450.030.8497815
GTAGCAT57600.030.8387558
AACACGT55550.030.6250465
GCGATTT39100.029.56606194
TAACACG58150.028.7708384
GTGGCGA108400.027.9638462
CGTGCGC61050.027.479589
CAGTAGC63400.027.3497456
TCACCCG48500.027.1309323
CGCAAAA48450.026.1332321
GCCAGTA66700.025.9987684
ACACGTG65500.025.9706866
TGGCGAC121050.025.0793443
TAACCCC49750.024.842219
Produced by FastQC (version 0.11.5)