FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14101530
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA190290.13494280407870635No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT185700.13168783812820312No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG164950.11697312277462091No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT154480.1095483965215122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA38250.033.9076924
CCCGTAT39950.033.7529751
TATCGAA48400.027.961975
CGTATCG58000.025.9264243
TGTCGAG9950.025.5030025
CCGTATC61550.024.6601142
CGCGTAT2002.2273525E-823.5739771
ATCGAAG73650.022.5866156
ACCCGTT31450.021.5160472
TCGACCG13300.021.21029330-31
CGGCATG44700.020.3945833
GTTCGAC14600.019.80232428-29
TATGCCG13100.019.73071548-49
CGCGTAA25850.019.3333951
TCGAAGG88800.018.9446957
TCGTATG16700.018.57279844-45
ACGACTT17750.017.5310691
GCGTAAC29300.017.3211522
CCGTTTA47350.016.9705834
ACGTACG7450.016.716652-53
Produced by FastQC (version 0.11.5)