FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12348837
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG245070.19845593556704977No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA185750.15041902326510587No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT145470.11780056696837118No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG124620.10091638589123818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT66400.037.7047961
GTATCGA67700.036.788434
TATCGAA72850.034.2522475
CGTATCG90000.030.492723
CCGTATC92850.029.5061442
ATCGAAG103650.026.2049066
TCGAAGG115350.023.8317157
CGTACGC23950.021.5829725
CGAAGGC118900.021.3416988
CCGTACG24500.021.290264
TGTCGAG9900.020.885345
CGCGTAA20650.020.7774161
CTTCGCG16150.020.3680556
TTGCACG63600.020.3185468
CGGCATG32300.019.7861923
GTACGCC27750.019.3048276
GAAGGCC134750.019.1801039
TCGACCG13250.019.16104530-31
GTTCGAC13300.019.08661328-29
CCCGTCG14000.018.85951
Produced by FastQC (version 0.11.5)