FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12348837
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC528240.42776497900166627No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC484500.3923446394182707No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT373030.3020770296020589No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG262020.2121819245002586No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA240540.19478757392295323No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA227760.18443842120517098No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT221550.1794096075606148No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT220350.1784378561317151No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC211680.17141695205791443No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA211210.1710363494149287No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT202810.16423408941263054No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG195310.15816064298200713No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC188800.15288889148022602No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT184100.14908286505036872No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG183100.14827307219295227No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT178930.14489623597752566No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA177870.14403785554866422No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC173480.14048286490460601No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT166750.13503295897419326No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC165800.13426365575964766No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT155770.12614143339976064No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA155320.12577702661392326No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG140840.11405122603853303No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA131840.10676309032178496No Hit
CTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCAT131500.10648776075026335No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC130910.10600998296438766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT26950.067.242051
GACCGTT31100.058.723282
ACCGTTG32000.056.5361253
CGCAAAA73450.054.2788431
CCGTTGA40800.044.2268834
ACATGCC127400.043.0053522
CACATGC147800.037.6109621
GCGATTT47800.037.2610294
ACCCGGC50500.036.848445
TAACCCC107050.036.6091279
TTAACCC109100.035.189038
TGCCTAT156400.034.8226625
CACCCGG54300.034.2697224
CGGCCCG56500.033.849798
CGTTGAC55100.032.919335
TCACCCG59550.032.3532073
CCCAAAC171650.032.27541
CATGCCT170700.032.153233
ACCGTAT63050.031.898773
GCCTATC171550.031.7473856
Produced by FastQC (version 0.11.5)