FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12310919
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG243080.19745073458772655No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA183560.14910340974544628No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT146300.11883759449639789No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG124470.10110536833196611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT71750.036.870991
GTATCGA74450.034.7151224
TATCGAA79200.032.6924255
CGTATCG98000.029.3937593
CCGTATC99400.028.6488342
ATCGAAG108350.026.3691066
TCGAAGG115350.024.728067
CGTACGC24900.023.967635
CCGTACG25450.023.4496654
CGCGTAA21950.022.9875721
CTTCGCG14800.022.225786
CGGCATG30650.021.6176933
GTACGCC28450.021.1421156
CGAAGGC124700.020.876728
TCGACCG12050.020.86707330-31
GCGTAAC24450.020.757052
TCCGTAC29200.020.5990813
TACGCCA29150.020.311927
CGATCCG12500.020.11467482-83
GTTCGAC13650.019.1087128-29
Produced by FastQC (version 0.11.5)