FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12431737
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG248110.19957790291091262No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA186390.1499307779757567No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT149610.12034521000564924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT65950.035.7550851
GTATCGA66950.034.324254
TATCGAA71100.032.5190775
CGTATCG90800.028.2067723
CCGTATC93000.027.6911092
ATCGAAG98150.025.6635826
CGTACGC25400.025.1621595
CCGTACG25700.024.6855834
TCGAAGG108750.023.550377
CGGCATG31800.022.0192173
TCCGTAC29100.021.9628473
TACGCCA29100.021.8007437
GTACGCC29200.021.7266946
CGCGTAA19300.021.0147271
TCGACCG11400.020.19975930-31
TTGCACG62750.019.6208618
GTTCGAC12050.019.49999228-29
CGAAGGC120900.019.395678
CTTCGCG15750.019.095996
GCGTAAC22100.018.7125572
Produced by FastQC (version 0.11.5)