FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12026925
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC431960.3591607996225136No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC366110.3044086497587704No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT293850.2443267917609863No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG218890.18199997089862954No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC198730.16523758150982068No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA195080.16220272430400956No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT185210.1539961378324052No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT176120.14643809618834408No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA173930.1446171818648574No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT172790.1436693086553712No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT167770.13949534066272135No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA166660.13857241148506372No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT161730.13447327558789965No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG152510.12680714313924799No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC151720.12615028363442857No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG147570.12269969256480773No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC142200.11823471086749107No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT140030.11643042589855677No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA134490.11182409468754483No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT129010.10726765154019002No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC125680.10449886400721714No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA123840.10296896338839727No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG121160.10074062987837705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21850.061.4234431
ACCGTTG27200.048.8901823
GACCGTT28150.047.509722
CGCAAAA67250.044.8155171
CCGTTGA33050.040.3785744
ACATGCC110600.037.12822
GCGATTT34550.034.95643694
TAACCCC89250.033.0108159
CACATGC127450.032.625831
ACCCGGC43800.030.6828735
TTAACCC94150.030.5934188
TGCCTAT138950.029.6582265
CCGTCAT67150.029.5942381
CGTTGAC45200.029.5246015
CATTCGG20300.028.7031634
ACCGTAT51000.028.65463
CCGTATG51200.028.1755584
GCCTATC146900.027.893236
CCCAAAC139000.027.7466031
CACCCGG48800.027.6354274
Produced by FastQC (version 0.11.5)