FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12133208
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA364080.3000690336801281No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT268110.2209720627883409No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT220430.1816749535654544No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA190630.15711426030115036No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA179830.14821306945368445No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA172990.14257564858362273No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT168480.138858577220468No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC153370.126405151877393No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC151980.12525953564795064No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT149360.12310017268310244No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA144540.11912760417525192No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA142350.11732264047562689No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA137290.11315226772672157No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC126610.10434997899978306No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG126160.10397909604780532No Hit
CTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGC123660.10191863520348451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA37850.037.4928551
GCGTAAC40900.035.731762
CACGGAA43550.032.48115594
CCCGTAT43150.032.34141
GTATCGA42600.031.3275244
CGTAACT47100.031.2277553
TGTCGAG13100.028.3382555
TATCGAA48000.028.2926675
TCGCGTA24500.027.6193682
CGGCATG41300.023.8938733
CCCGTCG20700.023.4592841
GTAACTA65500.023.2445434
CCGTATC75500.023.1533222
CGTATCG75350.023.012323
GTCGAGG16350.022.7052696
GAACGGG23450.022.6435229
ACTAGTT67650.022.2274957
TCGAGGG17650.021.8311967
AGGAACG25400.020.720147
GTCGGGA30100.020.5188331
Produced by FastQC (version 0.11.5)