FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12170894
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA368300.3026071872781079No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT264260.21712455962561175No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT225880.1855903107857155No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA192400.15808206036467No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA184110.15127072834583885No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA176070.1446648044096021No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT168720.138625806781326No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC157890.12972752864333548No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC152090.12496206112714481No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT148120.12170018077554534No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA145500.11954750406995575No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA144510.11873408806288181No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA139420.11455197950125931No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG128900.10590840738568588No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC126880.10424871007832291No Hit
CTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGC124000.10188240896683513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA39050.037.9254531
GCGTAAC43200.034.918692
CGTAACT49450.030.8854373
CACGGAA49100.030.63094994
CCCGTAT41950.030.4690911
GTATCGA42350.029.0726454
TCGCGTA25100.028.6453692
TATCGAA48550.025.843945
GTAACTA63900.024.6365934
GAACGGG21650.024.527029
TGTCGAG11250.024.2276975
ACTAGTT69300.023.0552527
CGCGTAT1252.3683475E-422.6393241
GTCGGGA28550.022.1371061
CGTATCG73150.021.649723
CCGTATC73250.021.556012
AGGAACG25000.021.4283667
CGGCATG43300.020.8377133
ACGTTCG7850.020.353826
ATCGAAG84050.019.624846
Produced by FastQC (version 0.11.5)