FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12000919
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA127930.10660016953701629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA16650.029.3533654
CCCGTCG27200.028.6057191
TTCGCGC12650.027.8611287
CCCGTAT23150.026.4807431
TATCGAA21000.023.0492465
GTTACGA8050.020.4320034
CTTCGCG17550.020.0827396
TCGACCG18050.020.04868130-31
GTTCGAC18050.019.91828728-29
ACGACTT24400.018.9397411
CCGGTAA10000.018.8624361
CGCGTAA24450.018.7081431
CGTATCG43100.018.208663
TTACGAC9100.018.0744675
CGCGTAT1350.00980720917.465221
CGATCCG20300.017.36256882-83
CCGTATC47600.017.1783352
GCGTAAC27850.016.536352
CCGTCGG47900.016.3839932
CGACGGG15100.016.34009730-31
Produced by FastQC (version 0.11.5)