FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12045686
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA891280.7399163484752964No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA413060.3429111467790211No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT372590.30931405650122373No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT330050.27399850867771247No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC319720.2654228244036911No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA272680.2263714993068888No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC272250.22601452503410763No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC253460.21041557948629908No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC253330.21030765703173734No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA238860.1982950576662882No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG229390.19043332193782903No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC202550.1681514859344665No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC188470.15646265393270256No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT146610.12171162356382192No Hit
CGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCC139840.11609135419933742No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG134010.11125144719860705No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT133960.11120993856223714No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG132540.11003109328933196No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC126360.10490062583401229No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT121910.10120635719709115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA18200.034.0816764
CCCGTAT23550.029.2283951
TATCGAA23950.027.0764645
TGTCGAG18100.027.0005845
GTCGAGG17900.026.7772226
TTCGCGC24800.026.5270717
CTTCGCG30000.021.6160436
TCGAGGG22300.021.4935577
ACGTTCG6350.021.460646
TCGACCG16200.020.750830-31
ACGACTT20600.020.5976831
ATCGCGT14200.020.51967294
GTTCGAC16200.020.31310528-29
CCCGTCG21950.020.1900021
AGTTTAC539850.020.04007194
GTTACGA6500.019.5195054
GTCGGTA9300.019.2638781
CGCGTAA17200.018.9131431
TGTGTCG25700.018.650363
CGGCATG38750.018.55413
Produced by FastQC (version 0.11.5)