FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12060784
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA893480.740814195826739No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA420100.3483189815852767No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT372170.30857861313161733No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT330150.27373842363813167No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC324010.2686475439739241No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA275090.2280863333594234No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC273440.22671826309135457No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC257100.2131702217699944No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC255020.21144562409873188No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA243530.2019188802319982No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG234940.19479662350308238No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC202950.16827264297246347No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC192380.15950870192186511No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT148830.12339993817980656No Hit
CGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCC138970.11522468191122567No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG134210.11127800647122112No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT133760.11090489639811144No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC130700.10836774790096565No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG129640.10748886639541841No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT123030.10200829398818519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA19100.031.9826534
TTCGCGC26700.030.6222527
TGTCGAG15450.028.2851835
CCCGTAT24350.026.5316731
TATCGAA24100.025.5422275
CTTCGCG32700.024.4296
GTCGAGG18350.024.0711216
CCCGTCG23200.023.3750691
GTCGGTA9450.021.4575311
GTTCGAC18450.020.37772828-29
CGGCATG39100.020.3102153
AGTTTAC537800.020.24000294
TCGACCG19050.019.73713930-31
CGCGTAA18000.019.1246451
CGATCCG19900.018.65764682-83
CGCTATC3055.005859E-918.553441
TCGGTAT11450.018.4676482
TCGAGGG23400.018.2736247
ACACGCG25950.017.9265618
ACGACTT23600.017.583841
Produced by FastQC (version 0.11.5)