FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10764566
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC253840.2358107145239297No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT228770.21252134085108493No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG196650.18268270174570903No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA173460.16113979885487256No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG166860.15500857164143914No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC161550.1500757206560859No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC159710.1483664088268863No Hit
CAAGAAAGTACCCCAAGTGTCAACTCCAACTCTTGTAGAGGTCTCAAGAA129860.12063654029340339No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC125010.11613101726535005No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT119440.11095663308674034No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT117970.10959104157102106No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC115730.10751014021373458No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC113280.10523415435420248No Hit
CACAAGCCCAAGGCAACAAAAGAGCAACTGAAAGCTGTTATGGATGATTT112760.10475108796768956No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG109720.10192700755422932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT19250.067.165221
ACCGTTG21500.060.567463
CGCAAAA50150.049.031281
CCGTTGA27000.048.5742574
GACCGTT26950.048.494912
TAACCCC68600.036.030649
TTAACCC72150.033.6724438
GTAGGTC34700.031.7027232
GCGATTT16550.028.9587894
AAATTAA85750.028.6128925
AGTAGGT38350.028.4423221
TCACATG65150.028.358832
ACATGCC97100.026.580512
GTTGACT75250.026.4287321
GCAAAAT98700.026.0067252
CCGTATG48450.026.0024
TTCGGCT53450.025.6260184-85
TAGGTCT44550.025.3257083
CGTATGG50800.025.166955
ATCTCCG55500.025.16616266-67
Produced by FastQC (version 0.11.5)