FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10768164
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC252200.23420891435160163No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT230450.21401048498146946No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG197120.1830581332156531No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA172000.15973010812242458No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG165480.15367522262848152No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC162080.15051776700280567No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC157220.14600446278492787No Hit
CAAGAAAGTACCCCAAGTGTCAACTCCAACTCTTGTAGAGGTCTCAAGAA127730.11861817854928658No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC124620.11573003531521252No Hit
CACAAGCCCAAGGCAACAAAAGAGCAACTGAAAGCTGTTATGGATGATTT116010.10773424327489813No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT115200.10698202590525181No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC114700.10651769419559362No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT113000.1049389663827557No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC111860.10388029008473497No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG109930.10208796968545428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT18150.062.6796841
ACCGTTG21100.054.133423
CGCAAAA51300.048.3843151
GACCGTT25800.044.2766382
CCGTTGA27350.042.2773064
TAACCCC66400.035.7411169
TTAACCC70050.033.610618
GTAGGTC37300.031.7599452
GCGATTT17250.031.32921694
ACATGCC94350.029.3468442
AAATTAA85200.028.7399045
ACCCGGC22700.028.5719855
AGTAGGT41850.028.4192811
TCACATG64200.026.4338382
CACATGC109050.026.0375021
GCAAAAT96950.025.9899232
TTCGGCT50500.025.31187684-85
ATCTCCG52600.025.21079866-67
TAGGTCT48000.024.873343
GTTGACT72400.024.7385641
Produced by FastQC (version 0.11.5)