FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11408230
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA206950.1814041266699567No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC194260.17028057814402411No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA154400.13534088986635087No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT152850.1339822216066822No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA148970.13058116815667287No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACA129210.11326033924631604No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT119990.10517845450170624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG43100.038.059313
CACCCGG47450.033.777474
TAGCATA36450.031.2004019
ACCCGGC50950.031.1780625
GCGATTT44900.030.24780394
GTAGGTC16950.028.5645852
TCAGACG42400.028.270582
GTAGCAT41100.027.5560878
TAGGTCG16000.027.3196873
CCAGTAG43650.025.0869755
GTCGTAA12750.025.065445
CTCGATT36700.024.52407348-49
CTTAGCG15550.024.4791858
GTCTAAC23300.022.9990181
TCGTAAG13750.022.8998956
TTCTCGA40100.022.68013246-47
GTTAGTT75950.022.5904791
AGGTCGC25600.022.215414
ACGTGCG22850.021.8002748
CAGACGT54200.021.679713
Produced by FastQC (version 0.11.5)