FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11461307
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA262480.2290140208267696No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA188950.1648590339653235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA12900.026.5894874
TGTCGAG19750.024.980385
GTCGAGG20650.023.4365676
ACGACTT23100.022.035871
GTTACGA9500.021.7623564
TGTTACG9350.021.608953
AGTTTAC166100.019.73590594
TTACGAC11300.018.2957865
GTCCGAT13300.018.0732781
TCGAGGG27600.017.8754527
ATCGCGT13250.017.3929894
GTCGTAT4355.456968E-1217.3360081
TGTGTCG28900.017.0713673
ACGTTCG13050.016.9224876
CCCGTAT23750.016.669941
TACGACT12450.016.6058146
TATCGAA22900.016.0042725
GTTCGAC22200.015.45219828-29
CGATCCG22700.015.43212182-83
CCGGTAA10150.015.3237941
Produced by FastQC (version 0.11.5)