FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12235600
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC369300.30182418516460163No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACA187460.15320866978325542No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACAC173020.14140704174703325No Hit
AGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCC132510.10829873483932133No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA125710.10274118147046322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG32650.034.1233063
TCGTAAG12700.031.0840236
GTCGTAA13200.029.9098995
TCAGACG40700.028.0678082
GGTCGTA15000.027.5774634
CACCCGG41450.026.8773674
ACCCGGC42250.026.365215
CTTAGCG13150.024.2999738
GTCTAAC30350.024.1656251
GCGATTT40700.023.89788694
ACGTGCG28850.023.7809758
GCGAACG6550.022.9664923
TAGGTCG15000.022.5645773
CTCGATT29350.022.50283848-49
CGCGGTC17850.022.3878731
CACGCCG32050.020.3868274
TTCTCGA32500.020.25117346-47
GGCGTAC53150.020.24490794
AACACGT36600.019.5196555
TAACACG36300.019.1654014
Produced by FastQC (version 0.11.5)