FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12222713
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA234160.19157776182750919No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA144550.11826343300378565No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA127780.1045430748476218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA3252.5465852E-1120.3055361
ATCGCGT7750.019.42279894
GTCGAGG16950.017.4634286
AGTTTAC185800.017.44363694
ACGACTT21950.016.965331
TGTCGAG17800.016.893465
CGACCGT17600.016.68543432-33
CGCTAGT2002.9291306E-416.4982471
CGATCCG16000.016.45849682-83
GTTCGAC16700.016.0385828-29
TCGACCG16900.015.7085630-31
GTTACGA9100.015.5399511
ACGTACG9350.015.32742152-53
GCGCGTT2801.8189894E-1215.11723690-91
CGCATAT6300.014.9643971
TTACGAC9750.014.4569042
TGTTACG9900.014.237863
GATCCGA20100.014.15421884-85
GAACTCT250200.014.1029929
CGCCCTT13150.013.9800461
Produced by FastQC (version 0.11.5)