FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13740153
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC278590.20275611195886978No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG262820.19127880162615366No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT230580.1678147252072084No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC207000.15065334425315352No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA206470.15026761346835074No Hit
CCCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTAC192010.1397437131886377No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT191020.1390231971943835No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG188700.13733471526845442No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA174340.1268835943821004No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG169250.12317912326012673No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA167790.12211654411708515No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC153930.11202932019752619No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT152750.11117052335588985No Hit
CAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGCTGC137850.10032639374539716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT23300.053.476251
ACCGTTG27300.045.977833
GACCGTT28900.043.6005672
CCGTTGA33500.036.9028744
CGCAAAA66750.032.965951
TAACCCC70450.030.2852299
GTAGGTC51600.029.2489662
TCGTAAG12000.028.1987196
CAACCCG31650.026.7405191
AACCCGT32100.026.5111622
GTCGTAA13000.026.3946635
GCGATTT20550.026.29734894
ACCCGTC32550.025.9968873
CGCGGTC15000.024.7632061
ATCGACC48450.024.1072642-43
AGTAGGT65050.023.5635811
CGTAAGG14750.023.2591977
CACATGC121000.023.1207871
GGTCGTA15100.023.035844
TAGAGGC109700.022.7555713
Produced by FastQC (version 0.11.5)