FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16268388
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC327090.20105864207320356No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT258690.15901391090500178No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG242770.14922806119450802No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC215220.13229337780731565No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG213170.13103326525037393No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA207940.12781844150754212No Hit
CCCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTAC196580.12083557387492848No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA185320.11391417514753151No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG177420.10905813163541464No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA176160.1082836234296846No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT173120.10641496871109787No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC172070.10576954520632284No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT171750.10557284470962951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT22250.047.542561
ACCGTTG28700.037.182333
GACCGTT29600.035.57612
CCGTTGA33150.031.9043754
GTAGGTC58500.030.3765132
CAACCCG34950.027.172791
GCATTCG33200.026.9034633
CCGTATG59100.025.690134
AACCCGT37550.024.9140952
CACATGC145500.024.3311161
AGTAGGT73600.024.1459351
TACCGTA63350.023.7468132
TAGGTCG15350.023.5816963
CATTCGG37500.023.5655634
TAGAGGC123850.023.4956423
CGTATGG64950.023.3748025
ACCGTAT65350.023.1634353
ACGACGT19550.023.076167
ACCCGTC40850.022.785883
CCTAGAG131750.022.5525931
Produced by FastQC (version 0.11.5)