FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16307403
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC328450.20141159202357362No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT257560.15794053780359754No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG243020.14902434188938607No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC215240.13198913401477844No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG212180.13011268563118236No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA208920.12811359356238392No Hit
CCCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTAC194020.11897663901480819No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA187410.11492326521887024No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA177250.1086929660105904No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG174570.10704954062887881No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT172010.1054797014582886No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT171610.10523441408788389No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC170290.10442496576554831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21350.049.1083221
ACCGTTG29550.035.7944643
GACCGTT29550.035.480212
CCGTTGA33700.031.3838464
GTAGGTC57750.029.3082122
CACATGC143650.025.3655451
CAACCCG38900.024.6563451
TAGAGGC120150.024.571223
CCGTATG56550.024.521274
AACCCGT39850.023.8320752
CCTAGAG130250.023.6795941
ACATGCC156050.023.1988122
AGTAGGT73300.023.1553521
CGCAAAA72550.022.4226441
CGAGCCG95450.022.30228294
ACCCGTC42500.022.2329963
TGCCTAT160150.022.1883875
GCATTCG32850.022.181313
ACGACGT20400.022.115337
CGTATGG63300.021.90535
Produced by FastQC (version 0.11.5)