FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13325197
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC269980.20260863685542513No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT238760.17917933971257613No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG200850.15072947889625946No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA188080.14114613089772707No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC184610.13854204181746807No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC166770.12515387202155437No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG162770.12215204022874859No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA159860.1199682075994824No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT151640.11379944326526654No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA151360.11358931503977014No Hit
CCCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTAC145220.1089815032378133No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG144390.1083586231408061No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT142870.1072179270595399No Hit
CAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGCTGC134280.10077149328448953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT17600.048.0836261
GACCGTT23950.035.726612
ACCGTTG24300.035.5960353
GTAGGTC49050.033.2595562
CCGTTGA27900.031.336464
AGTAGGT60650.027.2090681
GCATTCG24750.026.9713843
TAGAGGC100750.026.3629193
CCTAGAG107000.025.045431
TAGGTCT67600.025.03493
CATTCGG28350.023.5437974
CACATGC116300.023.4873371
CTAGAGG115850.022.6851542
GTTGACT101350.022.266651
CCGTATG53150.022.1978874
CAACCCG31250.022.1159041
CGAGCCG77400.021.91794694
AACCCGT32450.021.7321412
ACATGCC126300.021.5898972
GCCTATC122800.020.9727276
Produced by FastQC (version 0.11.5)