FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15432965
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1479930.9589408127342997No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG756400.4901196886016394No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA648290.4200683407238985No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC502420.32554988623378595No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC470010.3045493850339193No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA439060.2844949107316708No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT374880.24290860505418113No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA349740.22661879943355018No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA346940.22480450127373452No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT317890.2059811578656467No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA291190.18868052898454704No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG280270.18160476616126583No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG275150.17828719238331714No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACAC260440.1687556474079997No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT252370.16352658092595945No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC246770.1598979846063281No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT246680.15983966787976256No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA239150.15496050175711537No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC229680.1488242861951673No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA210970.13670088670582742No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT204300.13237896930369505No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC200210.1297287980631071No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG187240.12132470980138943No Hit
AGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCC181860.11783866548002928No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG181530.11762483748262242No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG176420.11431374334095877No Hit
CAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCCAAGGCAA173160.11220138191203051No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG164020.10627899434748928No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC155240.10058987368921007No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT155090.10049267914493425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG237750.066.2569963
CACCCGG251100.062.674154
ACCCGGC276700.056.9062545
CTCACCC285500.055.2755362
CCAGTAG142600.053.9247475
TAGCATA147750.052.191019
GTAGCAT155350.049.728968
CCTCACC329500.048.411971
GCGATTT290250.047.9026194
CAGTAGC162550.047.5015076
TGCCAGT173450.044.4475063
GCCAGTA176900.043.551274
CGGCCCG391700.040.513188
TCAGACG120750.039.8658032
GTCTAAC68900.038.143181
CGGACAC246950.036.93374312-13
CTGCCAG215650.036.0338862
GACACGG258500.035.29237414-15
CCCGGCC454100.035.2596056
CGGACAG259350.034.81394618-19
Produced by FastQC (version 0.11.5)