FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13663278
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA476790.3489572560845209No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT353350.2586129038727017No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT303150.22187208662518615No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA268150.19625597898249603No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA246120.18013246894339704No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG213450.1562216621809203No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC190500.13942481445521346No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT173010.1266240795217663No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC172710.12640451288482896No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT169640.12415761430017012No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG156470.11451863893862074No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC155270.11364037239087135No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC154490.11306949913483426No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC147260.10777794318464427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG72600.049.8524253
CGACCGT14850.048.4337431
GCGATTT72450.046.05489794
CACCCGG82550.043.728244
ACCCGGC82850.043.5101435
ACCGTTG18650.039.3167763
TCAGACG51950.035.381342
CCAGTAG55350.030.65335
GTCTAAC23800.030.6152481
CGGCCCG122100.030.1756258
CTCGATT70100.030.1368448-49
CCCGGCC127050.029.1116666
ATTCCGT71100.028.95131552-53
ACGTGCG25900.028.4875938
TAGCATA60900.028.397919
CGGACAC75750.028.1971712-13
CGGACAG75850.028.00448418-19
CGTTCTT76550.027.41170976-77
TTCTCGA78950.026.93848646-47
ATAGCTC78900.026.89352238-39
Produced by FastQC (version 0.11.5)