FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13696990
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA438070.31982939317324466No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA385160.28120046813204946No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT215640.15743604981824474No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA215510.15734113845450717No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG199790.1458641643163936No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC198250.14473982969980997No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA197230.14399514053817664No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC177480.12957591412419808No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC177020.12924007391404974No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT167750.1224721635921469No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC159030.11610580134759534No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA154690.11293722197358691No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT151740.11078346410415718No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC141330.1031832541310171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA28300.031.3792384
CCCGTAT32450.030.3583721
TGTCGAG17600.029.9000825
CCCGTCG21150.028.749231
GTCGAGG21400.025.688526
TATCGAA37650.024.3352975
GTTACGA10350.024.0603854
ACGACTT27450.022.83791
TCGAGGG25250.022.3299067
TCGACCG24750.021.54802730-31
GTTCGAC24300.021.46462828-29
TGTTACG12250.020.7121263
ACGTTCG6600.020.6452946
TTACGAC12350.020.5444185
CGCGTAA38550.020.2969421
AGTTTAC275150.019.9507994
GCGTAAC41450.019.497142
TGTGTCG28750.018.7943363
CGATCCG27900.018.62377482-83
AAGTTCG28300.018.59693726-27
Produced by FastQC (version 0.11.5)