FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13517239
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA345170.25535540209061924No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA212600.1572806399295004No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT171870.12714874687057023No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA161040.11913675566437791No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT151650.1121900707681502No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT150390.11125792774693116No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC143110.10587221251322108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT36950.035.8417931
GTATCGA35800.034.5188374
TATCGAA43850.029.0390995
CGTATCG74950.023.2587133
CCGTATC77250.022.3230022
TGTCGAG15350.021.4275765
CCCGTCG16600.021.0097791
AGTTTAC212900.019.30534694
TCGACCG11950.019.26895530-31
GTCGAGG17650.018.9015356
ATCGCGT11400.017.7380394
CGACCGT12050.017.15803332-33
GTTCGAC13450.016.94587328-29
CGTACGA11650.016.7394154-55
ATCGAAG97050.016.6550666
ACGTACG10800.015.88066152-53
CTTAACG141750.015.8117063
CGATCCG14250.015.67407682-83
CTTCGCG23450.015.6291436
TTAACGG144150.015.5484524
Produced by FastQC (version 0.11.5)