FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13490014
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT309370.22933260113740433No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT247010.18310581441946613No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC187930.1393104558675773No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT180500.1338026780402155No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA179570.13311327920045155No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA172280.12770928184359184No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT159250.11805028519614584No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT142840.10588573147514894No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT142220.10542613224863963No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG138800.10289092361208817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15000.051.4036981
ACCGTTG20000.038.5492063
GCGATTT41150.035.16953794
TCACCCG48350.032.5724073
ACCCGGC48750.032.2998775
CCGTTGA24300.032.1119234
GACCGTT26250.029.550352
CACCCGG61900.025.3641514
CGTATGG36600.025.17135
CCGTATG37050.024.7406394
GTCTAAC22050.024.5205971
CTTAGCG12100.024.4665728
TCAGACG35900.024.2261982
ATTCCGT36300.023.88740252-53
GTAGGTC25950.023.551252
CTACCGT40700.023.4500311
ACGTGCG22900.023.3930788
CTCGATT37500.023.06577148-49
TCGATTC34850.022.59183750-51
CGGCCCG73000.021.6288978
Produced by FastQC (version 0.11.5)