FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13535844
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT313880.23188801525785904No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT248500.18358663116980367No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC190210.14052319160888674No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT181590.1341549149059342No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA181380.13399977127395973No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA173750.12836288597888687No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT159180.11759887303665734No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT143250.10583012038259305No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT142450.10523909702269028No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG138270.1021509999671982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15600.047.310891
ACCGTTG21350.035.0054443
GCGATTT37550.033.41511594
GACCGTT24500.030.1244872
ACCCGGC46050.029.5970575
TCACCCG47050.029.3713593
CCGTTGA26450.028.4323644
ACGTGCG21200.026.6004288
GTCTAAC21050.025.9054991
CTCGATT33050.024.31769848-49
ATTCCGT33350.023.74482552-53
CACCCGG59950.023.3639224
GTAGGTC28400.023.3392492
TCAGACG36250.022.1755392
CCGTATG35350.021.8058554
CGTTCTT36900.021.52415376-77
CGTATGG36800.021.2002245
CGCAAAA51650.021.02461
TCGATTC33150.020.91151250-51
CGTGCGC28250.020.7938469
Produced by FastQC (version 0.11.5)