FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13544110
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA239970.17717664726585947No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG177780.13126000896330584No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT157380.11619811120848841No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA150370.11102242967607323No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT140770.10393447779145328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT32950.031.6122671
GTATCGA30750.030.559994
TGTCGAG14550.025.1883645
CCCGTCG14350.023.6482721
CGCGTAT1601.99221E-623.5661581
TATCGAA41950.023.4089895
ATCGCGT11400.023.10599994
CGTATCG63650.022.219613
CCGTATC67750.020.7362522
GTCGAGG19300.019.9629576
TCGACCG10700.017.12623830-31
AGTTTAC174900.016.40520394
TCGAGGG21900.016.305647
ATCGAAG87250.015.9940836
GTTACGA7400.015.923081
CGCGTTT3302.4900146E-715.7107731
CTTAACG82600.015.5292663
CTTACTT86800.015.0953731
TAAGCCG78550.015.05258766-67
GTTCGAC12050.015.01317328-29
Produced by FastQC (version 0.11.5)