FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13486385
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA361530.26807035391619033No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA186150.13802809277652983No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT163660.12135201538440435No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC135520.10048652770924159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG19200.027.8973275
GTATCGA15500.026.6753394
CCCGTAT21500.022.799551
AGTTTAC218200.021.40542694
CCCGTCG24650.021.2244991
TCGAGGG26650.020.9801587
TCGACCG16950.020.23635530-31
ATCGCGT15150.020.18041694
GTTCGAC17600.018.9563128-29
GTCGAGG29400.018.8579166
CGATCCG18200.018.73213482-83
ACGTACG14950.018.07184652-53
TATCGAA23700.017.8423945
CGCGTAT1851.6158365E-417.8343661
TGTGTCG32050.017.7384783
ACGACTT24250.017.687281
CGTACGA16500.017.65656354-55
CGACCGT19350.016.99771532-33
ACGTTCG7500.016.914596
GCTACGT17550.016.59898850-51
Produced by FastQC (version 0.11.5)