FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13486385
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA219730.1629272781401391No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT218010.16165191784158617No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA202000.14978068622540436No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG199820.14816424119584307No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA183520.13607797790141687No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG168000.12457007567261352No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA161880.12003216577311118No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC139560.10348214143374966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT50950.041.6888894
TCACCCG61000.038.0699733
ACCCGGC67550.034.3044625
CACCCGG70550.033.379724
GTCTAAC41050.031.4957331
TCAGACG48950.030.4458052
CTCGATT49050.027.02199648-49
CTTAGCG14100.026.3303458
ACGTGCG48650.025.9847158
TAACACG49450.025.8550014
CGACCTA10450.025.6389664
ACCGTTG11450.025.0443523
CGACCGT11750.024.807671
CGGACAC55200.024.1353112-13
ATTCCGT55150.023.9892652-53
GCGGGTC73550.023.5226382
GCCAGTA52200.023.5023864
AACACGT54650.023.3941785
CCAGTAG54000.023.0664735
TAGCATA56100.022.450139
Produced by FastQC (version 0.11.5)