FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17296899
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA270030.1561146885346327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA25800.028.0451034
CCCGTAT34750.024.6846391
TGTCGAG21000.020.3600145
AGTTTAC179000.018.89445594
GTCGAGG24650.018.2982856
CGTATCG73700.018.2966023
TCGACCG21500.018.0298830-31
CTTAACG84650.017.8724843
TATCGAA43200.017.8367675
CGCGTAT2654.3558248E-717.7854291
CCGTATC78100.017.3259682
CCCGTCG28950.017.0942861
TACGACG13500.017.053699
GTTCGAC22450.016.84945128-29
TTAACGG91950.016.4024734
AACGGTT93550.016.1721636
CTCCGAT44600.016.168391
ACCCGTT58900.015.7147312
CGATCCG24250.015.51372582-83
TTCGCGC17950.015.4434247
Produced by FastQC (version 0.11.5)