FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14479760
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT445150.30742912865959104No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG239480.16538948159361758No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA223830.15458129140261992No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG199410.13771637098957443No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA191630.1323433537572446No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG179640.12406282977065917No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA154960.10701834837041498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC96450.047.0745355
TCACCCG97300.046.720323
CACCCGG97850.046.3076634
GCGATTT90750.045.93113394
CGACCGT13500.040.398561
CGGCCCG125250.036.5077258
GCCAGTA70100.034.733494
CGACCTA19550.033.900734
CCAGTAG73900.033.072735
CAGTAGC78500.031.1917866
CTCACCC147600.031.1519472
TAGCATA80250.030.861169
CTCGATT85900.030.39404948-49
GTAGCAT82400.030.3412918
CGGACAC89800.029.33188412-13
ACCGTTG19300.028.9855693
CCGGCCC166950.027.8118067
TGACGAC25550.027.786111
ATTCCGT93200.027.70972452-53
CTTAGCG21450.027.6053288
Produced by FastQC (version 0.11.5)