FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13168886
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA24500.027.6230164
CCCGTAT29750.026.8707081
CGTACGC7450.024.602625
GTTACGA9550.023.6217754
CGTATCG33300.023.5693113
TATCGAA30300.023.1108915
CCGTATC37550.021.6524732
TTACGAC10550.021.3826625
CCGTACG8400.021.2607694
TCGACCG25800.021.03861630-31
CGGCATG57100.020.3299123
GTTCGAC26950.019.96655828-29
CGATCCG27700.019.3406882-83
TGTTACG11250.019.2167513
ACGACTT24550.018.9627291
CCCGTCG27650.018.8775221
CGCGTAT2302.2696204E-618.4005931
ATCGAAG50550.017.3850926
AAGTTCG32250.016.4666226-27
GTACGCC11800.015.9306866
Produced by FastQC (version 0.11.5)