FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12593193
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG132550.10525527560802093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA22300.027.396694
CCCGTAT28800.025.635151
CCCGTCG28250.024.6361031
CGTACGC10900.023.2823545
TATCGAA27550.023.0288265
CGTATCG33950.022.0098463
TCGACCG26050.021.55822230-31
GTTACGA7650.021.5013754
CGGCATG61100.021.3058173
ACGACTT24650.021.1755521
CGATCCG26100.021.0661982-83
GTTCGAC26750.020.7307228-29
CCGTACG12550.020.2213274
CCGTATC39800.019.4832082
TGTTACG9050.018.1751963
TTACGAC9150.017.976565
ATTCGCA55450.016.78034290-91
ATCGAAG51000.016.6785266
AAGTTCG34300.016.1676126-27
GCATGTA82300.016.1030775
Produced by FastQC (version 0.11.5)