FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14971621
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG218510.14594945998165462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA25950.030.7874724
CCCGTAT35250.028.813431
ACGACTT35650.028.358241
GTTACGA12450.027.5559844
CGTACGC22800.025.5592335
TATCGAA34150.024.633345
CCGTACG24100.024.3756074
TTACGAC13850.023.7524975
CCCGTCG43600.023.6188091
CGGCATG95850.023.2406713
TGTTACG14250.023.0858363
CGTATCG45050.022.4288023
TCGACCG48500.021.46312530-31
CCGTATC48900.021.3356112
GTTCGAC48600.021.27402128-29
GTACGCC28800.020.723456
CGATCCG50250.020.71510182-83
TACGCCA29550.019.879277
TCCGTAC30900.019.4676673
GCATGTA120600.018.4710675
Produced by FastQC (version 0.11.5)