FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14971621
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT547250.365524882041831No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG470580.31431466238692524No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG396310.26470747556326735No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC166550.11124379918513834No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC163830.10942702864305742No Hit
CAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGCTGC152180.10164564010804174No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT152100.10159220568033349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC111400.049.5837945
TCACCCG121900.045.4668123
CACCCGG129550.042.455814
GCGATTT121650.042.08423294
CGACCGT17800.041.9560661
CCAGTAG110050.041.143825
TAGCATA118500.038.923929
CGGCCCG145550.037.9823578
GTAGCAT124400.037.379418
GACCGTT20200.036.0409742
GCCAGTA127950.035.7182544
GTCGTAA17150.035.3299675
CAGTAGC132200.034.676566
GCGGGTC125850.033.813582
AGTAGCA137900.033.5497747
TCGTAAG18300.033.3664446
ACCGTTG22800.032.5490573
CGGGTCT139300.030.683613
CTCACCC182400.030.6434962
CTCGATT110450.029.7264748-49
Produced by FastQC (version 0.11.5)