FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14889158
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG217670.14619362626147162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA24750.028.4837844
ACGACTT33850.025.7004811
CCCGTAT32900.025.1561971
GTTACGA12350.023.9748124
TTACGAC12450.023.4047475
CGGCATG94600.022.8532683
CCCGTCG42700.022.4662461
TATCGAA33100.022.292225
TGTTACG13500.022.280653
CGTACGC22000.022.2173525
CCGTACG23200.021.0681784
TCGACCG49250.020.80202730-31
GTTCGAC49200.020.39339828-29
CGTATCG45400.020.2899913
CGATCCG50950.019.69234582-83
CCGTATC49000.018.9911922
TGTATTA119650.017.7136298
GCATGTA122050.017.713395
AAGTTCG58100.017.14823726-27
CATGTAT125600.017.0620826
Produced by FastQC (version 0.11.5)