FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14889158
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT546970.36736127052987144No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG468540.31468535695571237No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG397160.2667444324252587No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC168280.11302183776946957No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC165470.11113455844850327No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT152310.10229591223358633No Hit
CAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGCTGC150820.10129518405271809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC113600.048.6281135
CGACCGT15750.045.0356331
TCACCCG127550.043.567653
CCAGTAG105550.042.6794435
GCGATTT121300.042.193894
CACCCGG132550.041.7824554
TAGCATA113600.040.3574839
GTAGCAT119950.038.064248
CGGCCCG147300.037.8528948
GCCAGTA125350.035.8255964
CAGTAGC128500.035.2396
AGTAGCA131950.034.5312277
GACCGTT20600.034.4325832
GCGGGTC122550.032.542752
TCGTAAG16300.031.6996696
CTCACCC181150.030.8580512
CTCGATT112150.030.01439748-49
ACCGTTG24200.029.8927043
GTCGTAA17050.029.7548525
CTTAGCG23400.029.7096148
Produced by FastQC (version 0.11.5)