FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12227545
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA20450.029.8760364
CGTATCG23650.027.6220933
CCCGTAT28400.027.1551671
CGTACGC6950.027.0488075
CCGTATC28850.024.7610422
CCGTACG7800.024.101184
TATCGAA27450.022.9422155
ATCGAAG41750.018.6852056
TCGACCG12200.017.5267530-31
GTTCGAC12550.017.22525628-29
CGGCATG41450.016.8940853
TAATGCG16850.016.454759
TACGCCA12750.016.2174177
TTACGAC6200.015.9184415
CCCGTCG21500.015.52911951
TCCGTAC13600.014.5138723
GTACGCC13700.014.4070486
GCAAGCG49550.014.32091294
TCGGCAT58100.014.1557552
CGCTAAT3709.3272683E-713.9803461
Produced by FastQC (version 0.11.5)