FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12227545
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG512720.41931556988749585No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC166780.13639696275908206No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT149870.12256753093118856No Hit
CAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGCTGC135800.11106072396380468No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC133740.10937600311428008No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG128180.10482889247187396No Hit
CCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAAG124560.1018683636003793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTC124450.043.026382
CGCGGGT128350.041.499421
CGACCGT13100.039.0822941
CGGGTCT138850.038.25973
GTCTGTC153800.034.387726
GGTCTGT158200.033.342365
GCGATTT42600.032.3255494
TCGTAAG13450.031.7790136
TGCAACC159900.031.74410294
ACCCGGC51000.030.208335
GACCGTT16750.029.724592
GGGTCTG183200.029.2027054
CTTAGCG18800.028.2321288
GTCGTAA14900.028.0560685
ACCGTTG17950.027.9991053
ACGACGT17650.026.3458257
TAACCCC42850.026.088619
TAGCATA55550.025.5356989
TACACGC34800.024.8349572
CCAGTAG55250.024.7390925
Produced by FastQC (version 0.11.5)