FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12310509
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA20600.031.2287864
CGTATCG25500.029.2791633
CCCGTAT27250.027.241361
CCGTATC30550.026.4369852
TATCGAA27800.023.8163015
TCGACCG15050.018.40882530-31
GTTCGAC15500.018.17736828-29
CGTACGC6350.017.7475135
ATCGAAG44000.017.715516
CGATCCG16750.016.67962582-83
CTCGAGT23750.015.6279381
CCCGTCT32100.015.368181
CGCGTAT2155.207859E-415.29670051
GTCCGTT12350.015.2170771
CGCGATT3152.7349652E-614.9151511
CCGTACG7600.014.8285744
CGGCATG41550.014.6917683
TAATGCG19450.014.4849689
TTAACGC9200.014.2907857
ACGACTT21250.013.9289951