FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14092943
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT341400.24224890429202758No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC232130.16471364426862437No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT203450.14436303332809902No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG201800.1431922345815207No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA195590.13878577384439858No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT182830.12973159687085942No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA176190.125020018884629No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG169920.1205709836476313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21150.053.7870861
ACCCGGC73350.046.710955
CACCCGG76550.044.8833274
ACCGTTG25950.044.3789523
TCACCCG77350.044.3619773
GCGATTT75250.041.216794
CGGCCCG95650.036.0627828
GACCGTT36000.031.7298152
CGCAAAA66950.028.5067981
CTCGATT70100.028.49316248-49
CCCGGCC123350.028.4223616
TAACCCC68600.027.5391229
ATTCCGT72850.026.86779852-53
GTAGGTC38050.026.684732
CGGACAC74850.026.68346412-13
CCGGCCC132200.026.4478997
ACGGACA68800.026.36588918-19
AGCGCTC41950.026.3259499
CCAGTAG53400.025.9640275
CGGACAG81150.024.75660918-19
Produced by FastQC (version 0.11.5)