FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14105683
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT342040.24248382726309672No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC232400.164756290071172No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT204030.14464382901558187No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG201520.1428644043680834No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA196610.13938353782656254No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT184040.13047223590662005No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA176710.12527574878862655No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG169710.12031320993106112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21050.056.4894941
ACCCGGC72100.047.3859025
ACCGTTG26050.045.6460083
CACCCGG76500.044.7844124
TCACCCG76150.044.6229673
GCGATTT76300.040.2784294
CGGCCCG93350.036.7474988
GACCGTT36650.032.5730932
CGCAAAA69100.031.0840381
TAACCCC68100.030.9826419
CCCGGCC116650.029.8509966
AGCGCTC45500.028.401459
CTCGATT69350.027.95675748-49
CCGGCCC127300.027.5379227
CGGACAC72100.026.81926312-13
ATTCCGT72150.026.60906452-53
TTAACCC78000.026.5682648
GTAGGTC35850.026.351552
CCAGTAG51200.025.700295
ACGGACA70200.025.20216418-19
Produced by FastQC (version 0.11.5)