FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15620438
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA216020.1382931771823556No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG202290.1295034108518596No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA178030.11397247631596502No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA170250.108991822124322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT55050.037.5082661
GTATCGA53750.037.0698284
CGTATCG70000.030.8811133
CCGTATC74400.029.9390892
TATCGAA67150.029.4624775
ATCGAAG93300.022.4135956
GTTACGA10000.020.676924
CGCGTAT2751.3878889E-920.5711571
GTCGGGA35900.020.4852091
CCCGTCG24400.020.0934141
TCGAAGG107750.019.974757
GAACGGG30350.019.8191229
CGCGTAA26150.018.748731
ACGTTCG12050.018.3292186
TTACGAC11800.017.9210595
CGAAGGC112050.017.8661488
TGTCGAG14750.017.841415
TCGACCG17600.016.95502330-31
GCGTAAC29550.016.857052
GTTCGAC17800.016.36884728-29
Produced by FastQC (version 0.11.5)