FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15578457
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA443030.2843863163084765No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC340550.2186031646137997No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA313600.20130363360119685No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG308350.19793359509224823No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT264380.16970872018968244No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT243400.15624140439582687No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG239750.15389842524198644No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT211800.13595698213244098No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC206260.13240078911537903No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC178510.1145877284252221No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT177890.11418974292511767No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT176000.11297652906189619No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG166320.1067628199634919No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC165480.10622361380206011No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG160510.10303331068025542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT30350.061.797451
ACCGTTG36150.052.1403923
GACCGTT43750.042.761512
CCGTTGA50600.037.432324
GCGATTT53450.035.78088894
CGCAAAA78300.034.219171
ACCCGGC61850.033.3577045
TCACCCG64900.032.1570743
TAACCCC86700.031.0024959
CACCCGG66850.030.9345264
GTAGGTC52450.030.6498662
TTAACCC96700.027.6028658
TCACATG95750.026.6077732
CGTTGAC73950.026.0565555
AGTAGGT62850.025.877791
CTCGATT48100.025.79956648-49
CCGTATG69200.025.6051584
GTTGACT140100.024.9291251
TAGGTCT67300.024.0260093
CGGCCCG87450.024.0203888
Produced by FastQC (version 0.11.5)