FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004828882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12062589
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC282450.23415371277260627No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA282160.23391330003865674No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG272980.2263029934950117No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT224360.18599655513422533No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG216870.17978727452290713No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA203040.16832207414179495No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT186040.15422891387578572No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC165230.13697722769133558No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT147760.12249443299444257No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC144470.11976699197825608No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG137500.1139887962691923No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT136820.11342506985855194No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT135480.11231419722581944No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC129650.10748107226400568No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)