FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004828885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12073279
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC283460.23478294504748876No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA277640.22996238221613202No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG273390.22644221176368076No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT223050.1847468280986466No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG216670.17946243104296686No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA201690.16705486554232699No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT187180.1550365894799582No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC166850.13819775058623263No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT148610.12309000727971249No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC145120.12019932613169959No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT139430.11548643910241783No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT136590.11313413696477982No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG136050.11268686824846838No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC130150.10780004338506548No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA122470.10143888830863597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)